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JCU 2000 ;8(1) :78-86.
Transthoracic Echocardiographic Assessment of Adriamycin-induced Cardiomyopathy in Rats with a 15 MHz Transducer
Joo-Youn Choi, Ho-Joong Youn, Jin Hyoung Kang, Hui-Kyung Jeon, Hyou-Young Rhim, Ji-Won Park, Hee Yeol Kim, Jang Seong Chae, Jae Hyung Kim, Kyu Bo Choi, Soon Jo Hong
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
Corresponding Author: Ho-Joong Youn ,
ABSTRACT
Background:Adriamycin (doxorubicin) is one of the widely used drugs in the treatment of a variety of solid and hematologic malignancies. However, the adriamycin-induced cardiomyopathy limits the prolonged use of this effective drug. Transthoracic echocardiography is the excellent tool in early detection and follow-up studies of adriamycin-induced cardiomyopathy. The aim of this study was to assess the cardiac function and morphology using a 15 MHz high-frequency imaging in rats. Methods:Adriamycin was administrated intraperitoneally by six equal injections at a dose of 2.5 mg/kg over a period of 2 weeks for total cumulative dose of 15 mg/kg body weight in 12 male Sprague-Dawley rats (weight 367±39 g). Transthoracic echocardiography with a 15 MHz linear-array transducer was performed at baseline and additionally at 3 weeks to measure the left ventricular wall thickness and dimension from the parasternal short axis view with 2D guided M-mode and pulsed Doppler signals of mitral inflow. Within 2 days of echocardiography, the heart was harvested for electron microscopic evaluation after potassium-induced cardiac arrest. Results:1) The mortality rate during the experimental period was 0%. 2) Transthoracic echocardiography provided adequate 2D guided M-mode images and pulsed Doppler signals of mitral inflow in all rats. 3) In follow-up echocardiography, pericardial effusion was detected in 7out of 12 rats (58%). 4) Compared to baseline, end-diastolic dimensions were increased from 7.01±0.69 to 7.74±1.25 mm (p<0.001), end-systolic dimensions were increased from 4.13±0.69 to 5.22±1.12 mm (p<0.05), and interventricular septal and posterior wall thickness at end-systole and end-diastole were significantly decreased (p<0.05, respectively). 5) Fractional shortening was decreased from 43.0±6.8 to 32.7±8.0%, compared to baseline (p<0.05). 6) E/A ratio of mitral inflow changed significantly from 1.63±0.36 to 2.78±1.0, compared to baseline (p<0.05). Conclusion:Adriamycin administration at total cumulative dose of 15 mg/kg body weight over 2 weeks creates a reliable model of non-ischemic dilated cardiomyopathy in rats with a high success rate. Transthoracic echocardiography using a 15 MHz transducer provides adequate images for assessing the cardiac function and morphology in follow-up studies in adriamycin-induced cardiomyopathy of rats. These results suggest that transthoracic echocardiography using a 15 MHz Transducer is a promising tool for an assessment of adriamycin-induced cardiomyopathy in small animals.
Keywords: Adriamycin-induced cardiomyopathy, Rat, 15 MHz transducer
Volume 25, No 4
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